competent cell wiki

Competent cells - Pamela Stanley lab wiki有一對夫妻,老公正看著電視,啃著瓜子 忽然間老婆從廚房喊著:「老公可不可以幫我修電燈? 」 老公不耐煩的說: 「我又不是水電工」 沒多久老婆又喊:「老公可不可以幫我修冰箱?」 老公不耐煩的說: 「我又不是電器維修工」 又過了一會老婆又喊:「老公可不可Methods, protocols, techniques, tutorials and other information for biomedical students and researchers from the Laboratory of Prof. Pamela Stanley at the Albert Einstein College of Medicine in New York - Competent cells...

全文閱讀

Preparing chemically competent cells - OpenWetWare法庭裡面,一場詭異的氣氛,法官正在審理一件婚外情的案件;原告A太太懷疑丈夫A先生,和其年輕貌美的女秘書B小姐,兩人偷情,終於,會同徵信社人員、及派出所警察,一起「捉姦」,當場人贓俱獲…… 法院傳訊女兒A小妹妹出席作證。 法官:「小妹妹,不用怕,對於妳知Materials Plate of cells to be made competent TSS buffer LB media Ice Glassware & Equipment Falcon tubes 500μl Eppendorf tubes, on ice 200ml conical flask 200μl pipetman or repeating pipettor 5ml pipette Preparation Grow a 5ml overnight culture of cells i...

全文閱讀

TOP10 chemically competent cells - OpenWetWare一位優秀的商人傑克,有一天告訴他的兒子。傑克:「我已經決定好了一個女孩子,我要你娶她」兒子:「我自己要娶的新娘我自己會決定」傑克:「但我說的這女孩可是比爾蓋玆的女兒喔」兒子:「哇!那這樣的話...」在一個聚會中,傑克走向比爾蓋玆傑克:「我來幫你女兒介紹個好丈夫」比爾:「我女兒還沒想嫁人呢」傑克:「但Overview This protocol is a variant of the Hanahan protocol [1] using CCMB80 buffer for DH10B, TOP10 and MachI strains. It builds on Example 2 of the Bloom05 patent as well. This protocol has been tested on TOP10, MachI and BL21(DE3) cells. See Bacterial ...

全文閱讀

Natural competence - Wikipedia, the free encyclopedia有一對男女正在吃晚餐那個女生一直問那個男生:你愛不愛我 ?男生看了女生一眼又繼續吃晚餐女生很生氣又再問了一次:你愛不愛我?男生終於說:愛女生又問:那你要怎麼證明?忽然男生從口袋裡拿了三十元出來,且問女生 :你有沒有十元?女生拿了十元給了男生......男生就把四十元放在桌上過了一會兒 .....女生In microbiology, genetics, cell biology, and molecular biology, competence is the ability of a cell to take up extracellular ("naked") DNA from its environment. Competence may be differentiated between natural competence, a genetically specified ability o...

全文閱讀

Transformation/Preparation of Competent Cell Protocols剛開完高職同學會想起了當年學長告訴我們的教官的糗事當時是在學校裡不能抽菸的(現在好像也不行?)想當然爾 教官就是專門在抓抽菸的人物某日中午吃飽飯 教官接獲密報 要到五樓的廁所抓正在飯後菸的人抓耙子快 把風的人更快 馬上通報進廁所很快的全部有抽菸的就鳥獸散啦教官來了 開始用力敲每間隔間的門『誰躲在裡面Molecular Biology/DNA/Transformation/Preparation of Competent Cell ... Protocols How to Make Competent Cells (Sosnick Lab, University of Chicago) This 6-step protocol describes the CaCl2 method for making competent cells....

全文閱讀

Transformation (genetics) - Wikipedia, the free encyclopedia在無人的浴室裡,傳出陣陣的聊天聲。 『早知道就不要求上帝,說下輩子我想每天摸美女的頭髮!』梳子唉聲嘆道:『上輩子當個宅男就已經很不幸了!雖然這輩子可以每天撫摸美女的頭髮,但這不是我想要的。』 『是啊!我也一樣!早知道就不要要求說,想要每天和美女喇嘰(舌吻)!結果都是牙膏的味道,一點意思也沒有。』牙刷In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material (exogenous DNA) from its surroundings and taken up through the cell membrane(s). Transformation occur...

全文閱讀