木頭鉛筆的橡皮擦總是教人抓狂
Unspecific stimulation with PMA + ionomycin vs. PHA for intracellular cytokine staining of T cells哈哈...有點懷念耶 Dear Felix, I have an experience with PMA(10ng/ml) plus Ionomycin (1um) and also with PHA(0.3 ug/ml) on human PBMC activation for 48hr.I have measured different types of cytokine including IFN-gamma in cell couture supernatant by Elisa .in this experience...
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