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Targeted sequencing for gene discovery and quantification using RNA CaptureSeq : Nature Protocols : 看了很不忍心,但大自然的食物鏈就是如此,弱肉強食,是自古不變的道理... RNA sequencing (RNAseq) samples the majority of expressed genes infrequently, owing to the large size, complex splicing and wide dynamic range of eukaryotic transcriptomes. This results in sparse sequencing coverage that can hinder robust isoform assembly...
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