Full-Length cDNA Cloning and Determination of mRNA 5and 3Ends by Amplification of Adaptor-Ligated cD
5′-RACE amplification independent of the sequence, abu- n dance and size of the target cDNA, we developed a method that combines aspects of the standard adaptor-based technolo - gy for cDNA library construction (14). Our cDNA amplifica-tion protocol begin...
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