Staining for Analysis > FACS Facility | Laboratory Medicine | Yale School of Medicine
Harvest, wash the cells (single cell suspension) and adjust cell number to a concentration of 1-5x106 cells/ml in ice cold FACS Buffer (PBS, 0.5-1% BSA or 5-10% FBS, 0.1% NaN3 sodium azide*). *Do not add sodium azide to buffers if you are concerned with ....