Optimized expression vector for ion channel studies in Xenopus oocytes and mammalian cells using alf
Whole cell recording from tsA201 cells was performed as described previously for HEK293 cells [5]. Cells were plated in 35-mm culture dishes 24–72 h before transient transfection with pCEP or pUNIV cDNAs for rat α1, β2, and γ2L subunits. We used 200 ng of...