Perfluorooctane sulfonate blocked autophagy flux and induced lysosome membrane permeabilization in H
3.3. PFOS blocked autophagosome degradation in HepG2 cells To investigate the lysosomal turnover of LC3-II, we used chloroquine to prevent autophagosome-lysosome fusion (Yuan et al., 2009). The turnover assay allows the estimation of autophagy flux, which...