PINK1-mediated phosphorylation of the Parkin ubiquitin-like domain primes mitochondrial translocatio
(a) Phos-tag Western blotting detected phosphorylation of Ser65. HeLa cells were transiently transfected with Parkin WT and a series of alanine mutants for the candidate phospho-residues followed by treatment with or without 20 µM CCCP for 1 hr. Cell lysa...