miRNA extraction using Trizol - siRNA, microRNA and RNAi
I read your paper and I have a question: for the timepoint = 0 (i.e. the fresh RNA extraction). Was that ever frozen before assaying it with the qRT-PCR? Also, for your triplicates: are they PCR-only triplicates (i.e. same cDNA assayed 3 times in the qPCR...